Journal: Journal of the American Society of Nephrology
Article Title: Mesenchymal Stem Cells Prevent Progressive Experimental Renal Failure but Maldifferentiate into Glomerular Adipocytes
doi: 10.1681/asn.2007010044
Figure Lengend Snippet: Figure 1. Expression of surface markers on mesenchymal stem cells (MSC) in vivo by immunocytochemistry. Lewis MSC in passage 3, grown on coverslips, show no expression of surface markers CD31 (A) and CD34 (B) and rare (5%) expression of CD45 (C), whereas CD44 (D), CD73 (E), and CD90 (F) can be detected in 95% of the cells. Magnification, 400.
Article Snippet: Primary antibodies for the characterization of cultured MSC included mouse mAb against rat CD31 (clone TLD-3A12; Serotec), rat CD34 (ICO115; Santa Cruz Biotechnology, Santa Cruz, CA), CD44 (MCA643GA; Serotec), rat CD73 (BD Pharmingen, Heidelberg, Germany), rat CD90 (clone OX7; Mediagnost, Reutlingen, Germany), and a goat polyclonal antibody against rat CD45 (M-20; Santa Cruz) plus appropriate negative controls using irrelevant antibodies. aGAPDH, glyceraldehyde-3-phosphate dehydrogenase; PPAR- , peroxisome proliferator–activated receptor- ; RT-PCR, reverse transcriptase–PCR.
Techniques: Expressing, In Vivo, Immunocytochemistry